|
Antibodies
|
| Mouse Monoclonal Anti-CD38 |
Biolegend |
Biolegend Cat#303535, RRID:AB_2562819 |
| Mouse Monoclonal Anti-CD45 |
Biolegend |
Biolegend Cat#304045, RRID:AB_2562821 |
| Mouse Monoclonal Anti-CD25 |
Biolegend |
Biolegend Cat#356102, RRID:AB_2561752 |
| Mouse Monoclonal Anti-STAT3 |
BD Biosciences |
BD Biosciences Cat#610926, RRID:AB_398241 |
| Rat Monoclonal Anti-CD66 |
Invitrogen |
Invitrogen Cat#MA5-17003, RRID:AB_2538475 |
| Mouse Monoclonal Anti-IkBa |
CST |
CST Cat#4814, RRID:AB_390781 |
| Mouse Monoclonal Anti-CD11c |
Biolegend |
Biolegend Cat#337202, RRID:AB_1236381 |
| Mouse Monoclonal Anti-CD27 |
Biolegend |
Biolegend Cat#356401, RRID:AB_2561786 |
| Mouse Monoclonal Anti-Biotin |
Biolegend |
Biolegend Cat#409006, RRID:AB_10695474 |
| Mouse Monoclonal Anti-CD161 |
Biolegend |
Biolegend Cat#339938, RRID:AB_2564141 |
| Mouse Monoclonal Anti-CCR9 (CDw199) |
Biolegend |
Biolegend Cat#358902, RRID:AB_2562298 |
| Mouse Monoclonal Anti-CD20 |
Biolegend |
Biolegend Cat#302302, RRID:AB_314250 |
| Mouse Monoclonal Anti-Granzyme B |
BD Biosciences |
BD Biosciences, Custom Purified |
| Mouse Monoclonal Anti-STAT6 |
BD Biosciences |
BD Biosciences Cat#611567, RRID:AB_399013 |
| Mouse Monoclonal Anti-CD24 |
Biolegend |
Biolegend Cat#311102, RRID:AB_314851 |
| Mouse Monoclonal Anti-CD14 |
Biolegend |
Biolegend Cat#301802, RRID:AB_314183 |
| Mouse Monoclonal Anti-PD-1 (CD279) |
BD Biosciences |
BD Biosciences Cat#562138, RRID:AB_10897007 |
| Mouse Monoclonal Anti-Alpha4 (CD49d) |
BD Biosciences |
BD Biosciences Cat#555502, RRID:AB_395892 |
| Rat Monoclonal Anti-Beta7 |
BD Biosciences |
BD Biosciences Cat#555943, RRID:AB_396240 |
| Mouse Monoclonal Anti-CD8 |
Biolegend |
Biolegend Cat#301053, RRID:AB_2562810 |
| Mouse Monoclonal Anti-CD4 |
Biolegend |
Biolegend Cat#317402, RRID:AB_571963 |
| Mouse Monoclonal Anti-CD3 |
BD Biosciences |
BD Biosciences Cat#551916, RRID:AB_394293 |
| Mouse Monoclonal Anti-IgA |
Biolegend |
Biolegend Cat#411502, RRID:AB_2650697 |
| Mouse Monoclonal Anti-CD7 |
BD Biosciences |
BD Biosciences Cat#555359, RRID:AB_395762 |
| Mouse Monoclonal Anti-CD28 |
BD Biosciences |
BD Biosciences Cat#348040, RRID:AB_400367 |
| Mouse Monoclonal Anti-CD39 |
Biolegend |
Biolegend Cat#328202, RRID:AB_940438 |
| Mouse Monoclonal Anti-CD123 |
BD Biosciences |
BD Biosciences Cat#554527, RRID:AB_395455 |
| Mouse Monoclonal Anti-CD45RO |
Biolegend |
Biolegend Cat#304202, RRID:AB_314418 |
| Mouse Monoclonal Anti-CD16 |
Biolegend |
Biolegend Cat#302051, RRID:AB_2562814 |
| Mouse Monoclonal Anti-STAT5 |
BD Biosciences |
BD Biosciences Cat#611965, RRID:AB_399386 |
| Mouse Monoclonal Anti-CD45RA |
Biolegend |
Biolegend Cat#304143, RRID:AB_2562822 |
| Rat Monoclonal Anti-Fox P3 |
Thermo Fisher |
Thermo Fisher Cat#14-4776-82, RRID:AB_467554 |
| Mouse Monoclonal Anti-Beta1 (CD29) |
BD Biosciences |
BD Biosciences Cat#555442, RRID:AB_395835 |
| Rat Monoclonal Anti-Ki67 |
Thermo Fisher |
Thermo Fisher Cat#14-5698-82, RRID:AB_10854564 |
| Mouse Monoclonal Anti-IgG |
Milipore Sigma |
Milipore Sigma Cat#CBL101, RRID:AB_92830 |
| Armenian Hamster Anti-ICOS (CD278) |
Biolegend |
Biolegend Cat#313502, RRID:AB_416326 |
| Mouse Monoclonal Anti-CD62L |
Thermo Fisher |
Thermo Fisher Cat#BMS1015, RRID:AB_10596353 |
| Mouse Monoclonal Anti-CD19 |
Beckman-Coulter |
Beckman-Coulter, Custom Order |
| Mouse Monoclonal Anti-IgM |
BD Biosciences |
BD Biosciences Cat#555780, RRID:AB_396115 |
| Mouse Monoclonal Anti-CD56 |
BD Biosciences |
BD Biosciences Cat#559043, RRID:AB_397180 |
| Mouse Monoclonal Anti-HLA-DR |
Beckman-Coulter |
Beckman-Coulter, Custom Order |
| Mouse Monoclonal Anti-CD235 |
Biolegend |
Biolegend Cat#306615, RRID:AB_2562825 |
| Mouse Monoclonal Anti-CD71 |
BioXcell |
BioXcell Cat#BE0023, RRID:AB_1107669 |
|
Biological samples
|
|
|
| Peripheral blood from participants of: Phase 2 Influenza A Challenge Study Following Oral Administration of an H1N1 HA Ad-Vector Seasonal Flu Vaccine |
Liebowitz et al., 2020
|
ClinicalTrials.gov identifier NCT02918006
|
|
Chemicals, peptides, and recombinant proteins
|
| Biotinylated recombinant hemagglutinin A/California/07/2009/H1N1 (mutation Y98F) |
Gift from Barney Graham VRC/NIAID/NIH |
Gift from Barney Graham VRC/NIAID/NIH |
|
Critical commercial assays
|
| Smart Tube Proteomic Stabilizer |
Smart Tube Inc. |
Cat#PROT1 |
| 10X Thaw-Lyse Buffer |
Smart Tube Inc. |
Cat#THAWLYSE1 |
| Fc Block: Human TruStain FcX |
Biolegend |
Cat#422302 |
| 100% Methanol |
Thermo Fisher |
Cat#50-980-487 |
| Iridium DNA Intercalator |
Fluidigm |
Cat#201192B |
| 16% Paraformaldehyde |
Thermo Fisher |
Cat#50-980-487 |
| Four Element Normalization Beads |
Fluidigm |
Cat#201078 |
|
|
|
|
Software and algorithms
|
| Cell Engine |
Primity Bio |
https://cellengine.com
|
| Single Cell Debarcoder |
Nolan Lab |
https://github.com/nolanlab/single-cell-debarcoder
|
| Bead Normalization |
Nolan Lab |
https://github.com/nolanlab/bead-normalization
|
| R |
R Foundation |
https://r-project.org
|
| Fast PG |
Bodenheimer et al., 2020
|
https://github.com/sararselitsky/FastPG
|
| UWOT (v0.1.9) |
Becht et al., 2018 |
https://github.com/jlmelville/uwot
|
