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. Author manuscript; available in PMC: 2022 Dec 6.
Published in final edited form as: Dev Cell. 2021 Nov 5;56(23):3235–3249.e4. doi: 10.1016/j.devcel.2021.10.011

Figure 4: cAMP promotes neuron-specific assembly of electrical synapses.

Figure 4:

(A) Two-color labeling of UNC-7 and UNC-9 innexins in heterotypic VA→AVA gap junctions. VA→AVA gap junctions contain UNC-7 (AVA) and UNC-9 (VA). AVA promoter (Pflp-18) drives flippase resulting in expression of endogenous UNC-7::tagRFP (magenta) in AVA. VA promoter (Punc-4) drives expression of GFP::UNC-9 (green) in VAs. Schematic of dual-colored heterotypic gap junction. (B) Representative images of AVA::UNC-7::tagRFP (magenta arrows), GFP::UNC-9 (green arrows) and co-localized UNC-7 and UNC-9 (white arrows) puncta in wild-type, unc-4 and unc-4;goa-1(lof) in the ventral nerve cord of L4 larvae. VA cell soma denoted by dashed outline. Scale bar = 2.5 μm. (C) Violin plots for density of co-localized UNC-7::tagRFP and GFP::UNC-9 puncta in wild type, unc-4 and unc-4;goa-1(lof) VAs. Dashed line represents median. 2-way ANOVA. * = p < .05. **** = p <.0001. (D) Super resolution image of dual-colored gap junction. Scale bar = 200 nm. (E) 3D distance between centroids of UNC-7::tagRFP (magenta) and GFP::UNC-9 (green) images at dual-colored puncta. unc-4(e2323) was used in all experiments.