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. 2021 Nov 16;5(4):txab218. doi: 10.1093/tas/txab218

Table 4.

The effects of implanta and zinc sourceb on liver, muscle, and plasma parametersc

Implant treatments NoIMP IS/200 P-values
Zinc treatments ZnB ZnG ZnS ZnB ZnG ZnS SEM IMP Zn IMP × Zn
Steer (n)a 12 11 12 11 11 9
Liver, mg/kg DMe
 Znf 103 102 101 105 106 112 2.9 0.02 0.62 0.21
Cu 303a 314a 240b 298a 278ab 301a 18.3 0.62 0.19 0.02
Mne 9.0 8.5 9.1 8.5 8.3 8.8 0.30 0.19 0.19 0.86
Fe 167 153 156 164 147 155 6.3 0.53 0.04 0.92
Muscle, mg/kg DMe,g
Zn 118y 122y 123xy 124xy 133x 119y 3.7 0.16 0.14 0.09
Plasma, mg/Le
Cu 0.99 1.05 1.02 1.00 1.07 1.03 0.046 0.76 0.34 0.99
Fef 1.92 2.24 2.13 1.97 2.25 2.15 0.107 0.79 0.02 0.98
PUN, mg/dLe,f 8.20 9.02 9.12 7.96 7.83 7.37 0.485 0.01 0.78 0.30

a Implant strategies included no implant (NoIMP) or a Component TE-IS (IS/200; 80 mg trenbolone acetate + 16 mg estradiol; Elanco Animal Health, Greenfield, IN) on day 0 followed by a Component TE-200 (200 mg trenbolone acetate + 20 mg estradiol; Elanco Animal Health) on day 57.

b Cattle received 70 mg Zn/kg DM from ZnSO4 + 30 mg Zn/kg DM from basic ZnCl, Zn glycinate, or ZnSO4 (ZnB, ZnG, or ZnS, respectively). Total Zn supplementation was targeted at 100 mg Zn/kg DM to meet industry consultant recommendations (Samuelson et al., 2016).

c Liver and muscle samples were taken on days −5/−4, 14/15, 71/72, and 120/121, whereas blood for plasma trace mineral and PUN analysis was collected on days −1, 14, 56, 71/72, and 125.

d Unlike superscripts indicate differences between treatment means; a,b,c represent P ≤ 0.05 and x,y,z represent 0.05 < P ≤ 0.10.

e Data were analyzed using repeated measures of the mixed procedure of SAS and represent treatment means. No IMP × Zn × Day or Zn × Day interactions were observed (P ≥ 0.15) for all measurements except plasma Zn (IMP × Zn × Day; P = 0.06; Figure 1).

f An IMP × Day effect (P ≤ 0.01) for liver Zn (Figure 2A), plasma Fe (Figure 2B), liver Mn (Figure 3A), and plasma urea nitrogen (PUN; Figure 3B). No other IMP × Day effects were observed (P ≥ 0.27).

g A Day effect was observed (P < 0.0001; Figure 3).