Fig. 2.

Generation of NSCs with FAD mutation. A: Diagrams showing lentiviral vector constructs and infection. B–D: Culture observation and green fluorescence signals confirming the successful viral gene infection and expression. E–F: Images of western blotting of fusion protein of APP and flag showing the successful establishment and expression of the lentiviral vector; NSC-APP indicated the transfected cells with APP lentiviral vector constructs; NSC-NC indicated the transfected cells with negative control lentiviral vector constructs; NSC-C indicated normal NSCs. Scale bar (B–D): 100 μm.