Detection of proteomics in a 2-day medium of 3D bioprinted FAD NSCs and control groups. Venn diagram of proteins found in 3D and control groups (A). Gene ontology (GO), Kyoto encyclopedia of genes and genomes (KEGG), and protein–protein interaction network (PPI) analysis of the differences of proteins between 2D and 3D (B), 3D and MIX (C), and 2D and MIX (D). GO analysis listed 10 significant enrichment categories in a biological process, cellular component, and molecular function (P-values set to 0.05). KEGG analysis also listed the 10 most significant pathway entries.