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. 2021 Sep 23;11:192–205. doi: 10.1016/j.bioactmat.2021.09.023

Fig. 8.

Fig. 8

Evaluation of amyloid-β and p-tau levels in 3D bioprinted FAD NSCs. A: Immunofluorescence (IF) analysis of Aβ (red) with a paraffin section. B: Immunohistochemical (IHC) staining of Aβ plaques (brown) with a paraffin section. C: Comparative APP-related gene expression of cells after removal of the shell among 3D bioprinted NSC constructs (APP-3D-P) and control groups (APP-MIX-P and APP-2D-P). Relative gene expression was normalized to ACTB and compared to 2D. Mean ± S.D.; n = 3. Two-way ANOVA. *p < 0.05; **p < 0.01; ****p < 0.0001. D: Different expression of fusion protein of APP and flag was detected via western blotting (WB) analysis among 3D bioprinted NSC constructs (3D-P) and control groups (MIX-P and 2D-P). E: Comparative protein relative expression analysis based on the results of WB among 3D bioprinted NSC constructs (3D-P) and control groups (MIX-P and 2D-P). The relative value was normalized to GAPDH and compared to 2D with ImageJ and Prism 8 software. Mean ± S.D.; n = 3. One-way ANOVA. ****p < 0.0001.