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Primers and Gene Fragment Used for Evaluating Artemisinin Resistant P. falciparum Markers
| Gene Fragment | PCR Primer Sequence | Assessed Codons |
|---|---|---|
| K13-propeller (1st round PCR) | 5ʹ_CGGAGTGACCAAATCTGGGA-3 | T474I, M476I, A481V, Y493H, T508N, P527T, G533S, N537I, R539T, I543T, P553L, R561H, V568G, P574L, A578S, and C580Y |
| 5ʹ_GGGAATCTGGTGGTAACAGC-3 | ||
| K13-propeller (2nd round PCR) | 5ʹ_TCAACAATGCTGGCGTATGTG-3 | |
| 5ʹ_TGATTAAG GTAATTAAAAGCTGCTCC-3 |
