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. 2021 Oct 8;11:90–106. doi: 10.1016/j.bioactmat.2021.10.006

Fig. 4.

Fig. 4

Immunofluorescent staining and MOD values of BMP-2, Runx-2, COL-I, OCN, OPN in MC3T3 culturing on BCP-micro (A), BCP-nano (B) and BCP-control (C) for 14 days, and semi-quantification by optical density (D). For BMP-2, Runx-2, COL-I and OCN: red represents target protein, green represents cytoskeleton, blue represents cell nucleus; For OPN: green represents target protein, red represents cytoskeleton, blue represents cell nucleus. Values are expressed as the mean ± SD (n = 3), * refers to p < 0.05, and ** refers to p < 0.01.