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(A) Expressions of osteogenic genes (Runx-2, Osx, Col-I, Alp, Opn, Ocn) in MC3T3 cultured on BCP-control, BCP-micro and BCP-nano for 4, 7, 14 days; (B) representative Western blot analysis for COL-I, BSP, OPN, OCN and β-actin protein expressions of the respective groups co-culturing with MC3T3 for 14 days; (C) the protein expression level of COL-I, BSP, OPN and OCN to the β-actin level. Values are expressed as the mean ± SD (n = 3), * refers to p < 0.05, and ** refers to p < 0.01.
