Representative images of sham and pilocarpine treated animals, stained against Kir4.1 (green). Expression of Kir4.1 at 1 DPSE (A), 7 DPSE (B), 30 DPSE (C). (D) Representative current recordings in control ACSF (pre- Ba2+), ACSF to 100 mM Ba2+ (post- Ba2+ ) and Ba2+ -sensitive traces (Ba2+-sensitive) in response to a voltage step protocol (−180 mV to +80 mV, Δ20 mV) for astrocytes in the CA1 of Sham and LiPilo animals 7–14 DPSE. (E) I-V curve of control ACSF (pre- Ba2+), ACSF to 100 mM Ba2+ (post- Ba2+ ) and Ba2+-sensitive traces (Ba2+-sensitive) in response to a voltage step protocol (−180 mV to +80 mV, Δ20 mV) for each Sham and LiPilo animals. Insets – conductance as measured by the slope between −140 mV to −60 mV (Sham n=19 cells; LiPilo n=18 cells, p<0.05) Error bars represent S.E.M.