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Oligonucleotide primers used for RT-PCR analysis of DLK1 (target gene) and ACTB (reference gene) in Kermani lamb tissues.
| Studied genes | Sequence of used primer (5′-3′) | GenBank No. | Tm (°C) | Size of amplicon (bp) | Efficiency (%) |
|---|---|---|---|---|---|
| DLK1 (Target gene) | F: CGTCTTCCTCAACAAGTGCGA | NM-174037 | 57 | 102 | 98 |
| R: TCCTCCCCGCTGTTGTAGTG | |||||
| beta actin (ACTB) (reference gene) |
F: CCTGGCACCCAGCACAAT | NM_001101.3 | 57 | 144 | 99 |
| R: GGGCCGGACTCGTCATAC |
