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. 2021 Dec 2;7(12):e08522. doi: 10.1016/j.heliyon.2021.e08522

Figure 2.

Figure 2

Standardisation of CUS paradigm in rats. (A) The body weight of control and CUS group were measured every week, before the start of stress protocol till the end. The CUS group animals gained less weight compared to control group. (B) Body weight assessment revealed significantly less weight variation (%) in CUS rats depicting weight loss as well as less weight gain in rats exposed to 8 weeks of CUS. (C) In force swimming test the immobility time was found significantly increased in CUS rats compared to control. (D) Elevated plus maze test revealed increased time spent in closed arms by CUS rats compared to control group. (E) Novelty suppressed feeding test revealed increased latency to the first bite by CUS rats, revealing depressive behaviour. (F) Serum corticosterone level was significantly elevated in CUS rats after 8-week of stress paradigm. All parameters were compared between the CUS and control groups using unpaired t-test (∗P<0.05, ∗∗ P<0.01, ∗∗∗ P<0.001, ∗∗∗∗ P<0.0001).