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. 2021 Nov 12;297(6):101400. doi: 10.1016/j.jbc.2021.101400

Figure 11.

Figure 11

The effect of nuclear receptor corepressors on expression of miR-199a-5p.A and C, transfection of a miR-199a-5p mimic into Huh-7 cells led to a significant decrease in NCoR1 mRNA and protein levels, which could be blocked by expression of an miR-199a-5p mimic inhibitor. B and C, the expression of the miR-199a-5p mimic had no effect of SMRT mRNA and protein levels. D, in vivo ChIP analysis of the miR199a-2 promoter was done using mouse liver after common bile duct ligation (CBDL), after sham surgery, and after treatment with obeticholic acid. There was marked depletion of NcoR1 binding at miR199a-2 FXRE after 4 days of CBDL compared with sham-operated mice as determined by quantitative RT-PCR. In mice treated with OCA NcoR1 recruitment to the FXRE was maintained at levels found in sham-operated mice. There was no change in recruitment of a related corepressor, SMRT, under any of the experimental conditions. ∗, p < 0.05 versus all other treatments using one-way ANOVA and Tukey's correction. Each panel represents the mean ± SEM of three separate experiments.