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. 2021 Nov 12;297(6):101400. doi: 10.1016/j.jbc.2021.101400

Figure 4.

Figure 4

miR-199a-5p regulates the expression of ABCB11 by targeting the 3′-UTR of its mRNA. Huh7 cells were transfected with an ABCB11-3′ UTR-luciferase construct in the presence of a miR-199a-5p mimic with and without a mimic inhibitor. Luciferase activity was measured 48 h after transfection. A, expression of a miR-199a-5p mimic significantly repressed luciferase expression. The activity of the mimic was completely reversed by a mimic inhibitor. B, site-directed mutagenesis of the miR-199a-5p binding site in the ABCB11-3′ UTR completely abrogated the inhibitory effect of the miR-199a-5p mimic on luciferase expression. C, a miR-let7a-5p mimic had no effect on expression of an ABCB11-3 UTR-luciferase construct expressed in Huh7 cells. D, a miR199-5p mimic did not suppress expression of an ABCC2-3′UTR -luciferase construct expressed in Huh-7 cells. Statistical analysis was performed using one-way analysis of variance and Tukey’s correction for multiple comparisons. Each panel represents the mean ± SEM of three separate experiments. ∗ p ≤ 0.05.