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. 2021 Nov 12;297(6):101400. doi: 10.1016/j.jbc.2021.101400

Figure 5.

Figure 5

The effect of an miR-199a-5p mimic on endogenous ABCB11 mRNA and protein levels in Huh-7 cells.A, transfection of an miR-199a-5p mimic but not a let7a-5p mimic into Huh-7 cells led to a significant decrease in ABCB11 mRNA levels. B, the effect of a miR-199a-5p mimic was blocked by expression of a miR-199a-5p mimic inhibitor. C, the miR-199a-5p mimic also suppressed endogenous ABCB11 protein levels. D, the miR-199a-5p mimic had no effect on ABCC2 protein levels in Huh-7 cells. Statistical analysis was performed using one-way analysis of variance and Tukey’s correction for multiple comparisons. Each panel represents the mean ± SEM of three separate experiments. ∗ p ≤ 0.05.