Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Dec 11;14:53. doi: 10.1186/s13072-021-00427-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 1 — Formaldehyde fixation condition, DNA damage response pathways markers and one-pot UniNicE-seq labeling efficiency in HCT116 cells. A Left panel, representative visualization of ADPribose and phosphoSer139 histone H2A.X using 561 nm (red) and 488 nm (green) laser of cells fixed with different concentration of formaldehyde as indicated in Y axis. The antibodies used for probing are indicated on top. DNA content of nuclei is revealed by DAPI (blue) staining. Right panel shows quantification of fluorescence, represented as mean pixel intensity from anti-pH2A.X and anti-ADPribose staining. B Visualization of NicE-view using fluorescein-dATP (green) on cells differently fixed with different formaldehyde concentrations (Y-axis) at different time intervals indicated on the top. Left panel shows Fluorescein-12-dATP labeling in a one-pot UniNicE-seq reaction. The labeling efficiency is shown at right panel