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. 2021 Dec 11;9:88. doi: 10.1186/s40364-021-00336-2

Fig. 1.

Fig. 1

Flow chart of single-cell sequencing technology. SCS of tumor tissues can be summarized in the five steps: (1) simple collection; (2) single cells are isolated or add a unique barcode to each cell; (3) amplified; (4) and sequenced; followed by (5) bioinformatics and statistical analyses