Table 3.

Technical characteristics of single-cell transcriptomic sequencing technologies

	Transcript coverage	Amplification	UMI	Advantages	Disadvantage	Ref
Tang2009	Nearly full-length	PCR	No	Sensitive, accurate	Less cell flux, expensive	[4]
Smart-seq	Full-length	PCR	No	Sequence coverage is better	Amplification of non-chain specificity	[23]
Smart-seq2	Full-length	PCR	No	Increased output, simplified steps	Less cell flux, more expensive	[23]
CEL-seq2	3′-only	IVT (In vitro-transcribed)	Yes	Reduced contamination between samples	Existence Sequence preference	[21]
Drop-seq	3′-only	PCR	Yes	Low cost, rapid library preparation, single cell high throughput, multiple possibilities	Needed microfluidic platform, low sensitivity of single cell genes	[24]
MARS-seq	3′-only	IVT	Yes	High throughput, Strictly control amplification bias	expensive	[22]
10x Chromium	Full-length	PCR	Yes	Simple and convenient, High throughput	require large initial cell count	[27–29]
Quartz-seq	Full-length	PCR	No	reduce PCR by-products、Reducing contamination of small fragments	Amplification bias	[30]