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. 2021 Dec 10;18:287. doi: 10.1186/s12974-021-02342-5

Fig. 2.

Fig. 2

Recurrent NTG injection induced hyperalgesia and upregulated the expression of CGRP, c-Fos. A The periorbital and paw mechanical threshold and thermal withdrawal latency during the injection of NTG. B, C The protein expression of CGRP and c-Fos in the TNC. D Counting of c-Fos was restricted to laminae I and II of the TNC region. E, F Immunofluorescence staining images of CGRP and c-Fos in the TNC. (n = 6–8 in each group; scale bar = 20/100 μm; *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 compared with the Sham group)