Table 2.
Antibodies used in Western blotting and immunofluorescence analysis
| Antibody | Manufacturer | Catalog number | Host | Dilution |
|---|---|---|---|---|
| For Western blotting analysis | ||||
| SIRT1 | Abcam, UK | ab110304 | Mouse | 1:3000 |
| CGRP | Abcam, UK | ab189786 | Rabbit | 1:3000 |
| c-Fos | Abcam, UK | ab134122 | Rabbit | 1:1000 |
| p-ERK | CST, USA | 4370 T | Rabbit | 1:1000 |
| ERK | CST, USA | 4695 T | Rabbit | 1:2000 |
| p-CREB-S133 | Abcam, UK | ab32096 | Rabbit | 1:3000 |
| CREB | CST, USA | 9197 T | Rabbit | 1:1000 |
| Iba1 | Abclonal, China | A1527 | Rabbit | 1:1000 |
| iNOS | Proteintech, USA | 18985-1-AP | Rabbit | 1:1000 |
| GAPDH | ZEN-BIOSCIENCE, China | 200306-7E4 | Mouse | 1:5000 |
| β-actin | ZSGB-Bio, China | TA-09 | Mouse | 1:3000 |
| Anti-rabbit IgG (HRP) | ZEN-BIOSCIENCE, China | 511203 | Goat | 1:5000 |
| Anti-mouse IgG (HRP) | ZEN-BIOSCIENCE, China | 511103 | Goat | 1:5000 |
| For immunofluorescence staining | ||||
| SIRT1 | Abcam, UK | ab110304 | Mouse | 1:50 |
| Iba1 | Wako, Japan | 019-19741 | Rabbit | 1:400 |
| NeuN | CST, USA | 24307 T | Rabbit | 1:400 |
| GFAP | Wanleibio, China | WL0836 | Rabbit | 1:50 |
| CGRP | Santa Cruz, USA | sc-57053 | Mouse | 1:100 |
| c-Fos | Novus Biologicals, USA | NBP2-50057SS | Rabbit | 1:5000 |
| Alexa Fluor 488 goat anti-mouse IgG | Beyotime, China | A0428 | Goat | 1:500 |
| Cy3-conjugated goat anti-rabbit IgG | Beyotime, China | A0516 | Goat | 1:500 |