Fig. 9.

Decrease in apoptosis with JZ induced by HCoV-229E infection in Huh-7 cells. (A) Percentage of apoptotic cells in Huh-7 cells were detected by flow cytometry and was analyzed by SPSS ver. 25.0; (B) Huh-7 cells were stained by JC-1 and the change of ΔΨm was detected by fluorescence microscopy; (C) The protein expressions of caspase 9, caspase 3, Cleaved caspase 9, Cleaved caspase 3, PARP and p-PARP in Huh-7 cells were detected by western blot analysis; (D) The quantitative analysis of the caspase 9, Cleaved caspase 9, caspase 3, Cleaved caspase 3, PARP and p-PARP proteins was analyzed by Image J. The values were presented as the means ± SD of three individual experiments. * p < 0.05; ** p < 0.01; *** p < 0.001, when compared to the viral control.