a, Self-assembly procedure of single-chain CD98 antibody-functionalized siRNA-loaded NPs. b, Specificity of scCD98-functionalized FITC-siRNA–loaded NPs (green) against in vitro colonic epithelial cells (Colon-26 cells; top), macrophages (RAW 267.4; bottom). c-d,
In vivo therapeutic efficacy of siCD98 NPs as measured by body weight changes (c), CD98 mRNA levels (d; left), and TNF-alpha mRNA levels (d; right) in the colon of DSS-induced colitis mice. Reproduced with permission from [204].