Fig. 6. CD82 overexpression protected against axonal transport deficits through a mTORC1-dependent mechanism.

a Western blot analysis of phosphorylated-pS6K and total pS6K in HEK-293T cells in CTR group and H₂O₂ groups with or without Cd82 plasmid transfection. b Statistical analysis of the data shown in (a), n = 4. c Western blot analysis of phosphorylated pS6K and total pS6K in HEK-293T cells in CTR group and Cd82 plasmid transfected groups with or without Rapamycin. d Statistical analysis of the data shown in (c), n = 4. e Co-immunofluorescent staining of p-S6 (red) with RGC marker Tuj1 (green) in retina flat mount. Scale bar, 50 μm. f Representative images showing anterogradely transported CTB-555 in ONH and proximal ON in Cd82 overexpression groups with or without rapamycin 2d after AOHT, n = 5 eyes. Scale bar, 100 μm. g Representative images showing CTB-555 transported to SC from three layers as Rostral, Middle, and Caudal, n = 5 mice. Scale bar, 200 μm. h Immunostaining of Synaptophysin within ONH, n = 5 eyes. Scale bar, 100 μm. i Schematic drawing of Cre induced conditional knockdown of Raptor in RGCs in Rptor^fl/fl mouse. j Co-immunofluorescent staining of p-S6 (red) with RGC marker Tuj1 (green) in ganglion cell layer (GCL) of retina sections in vehicle or Cd82 overexpression groups with or without knockdown of Raptor by Cre. Scale bar, 50 μm. k Statistical analysis of p-S6 and Tuj1 co-labeling in GCL shown in (j), n = 5 eyes. l Immunostaining of Synaptophysin within ONH in Cd82 overexpression groups with or without knockdown of Raptor 2d after AOHT, n = 5 eyes. Scale bar, 100 μm. m Representative images showing anterogradely transported CTB-555 in ONH and proximal ON, n = 5 eyes. Scale bar, 100 μm. n Representative images showing CTB-555 transported to SC from three layers as Rostral, Middle, and Caudal, n = 5 mice. Scale bar, 200 μm. Statistical tests in (b), (d) and (k) using one-way ANOVA, *P < 0.05, **P < 0.01, ***P < 0.001.