Table 2.

Summary of target genes, primer sequences, and polymerase chain reaction amplification protocol for gene transcript analysis.

Target Gene Name	Primer Sequences (F: Forward, R: Reverse)	PCR Amplification Protocol
Collagen Type 1	F: 5′-GGC GAG AGA GGT GAA CAA GG-3′	Activation: 95°C for 15 minutes Cycling (45 cycles): 1) Denaturation: 94°C for 15 seconds. 2) Anneal: 51°C for 30 seconds. 3) Extension: 72°C for 30 seconds.
	R: 5′-GGC GAG AGA GGT GAA CAA GG-3′
Collagen Type 3	F: 5′-AGA AGG CCC TGA AGC TGA TG-3′
	R: 5′-TGT TTC GTG CAA CCA TCC TC-3′
Tenascin C	F: 5′-TTC AGG AAC CCA GAG GAA GC-3′
	R: 5′-TCC GGT TCG GCT TCT GTA AC-3′
Scleraxis B	F: 5′-CTG GCC TCC AGC TAC ATC TC-3′
	R: 5′-CTT TCT CTG GTT GCT GAG GC-3′
GAPDH	F: 5′-CCC ACT CCT CCA CCT TTG AC-3′
	R: 5′-CCA CCA CCC TGT TGC TGT AG-3′