Skip to main content
. Author manuscript; available in PMC: 2022 Dec 16.
Published in final edited form as: Cell Chem Biol. 2021 Jun 23;28(12):1669–1678.e5. doi: 10.1016/j.chembiol.2021.05.016

Figure 1. A high-content screen identifies genotoxic agents as activators of intracellular H2S levels.

Figure 1.

(A) Schematic of high-content screen for small molecule modulators of intracellular H2S levels. (B) Scatter plot of the primary screening data. The dotted line indicates the cut-off for activator hits. Genotoxic hits are highlighted in red. (C) Intracellular H2S levels in HeLa cells treated with representative genotoxic hits for 20 h (n=3). (D) Representative P3 fluorescence images of HeLa cells treated with 30 μM teniposide (Ten) for 6h prior to staining with P3. Scale bar, 100 μm.