KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti- phospho-H12AX (Ser139)	Cell Signaling Technology	Cat# 2577
Anti- vinculin	Cell Signaling Technology	Cat# 13901
Anti- PARP-1	Cell Signaling Technology	Cat# 9542
Anti- β-tubulin	Cell Signaling Technology	Cat# 2128
Anti- ATG5	Cell Signaling Technology	Cat# 12994
Anti- ATG7	Cell Signaling Technology	Cat# 8558
Anti- AMPKα1	Cell Signaling Technology	Cat# 2532
Anti- phospho-AMPKα1 (Thr172)	Cell Signaling Technology	Cat# 2535
Anti- ATF4	Cell Signaling Technology	Cat# 11815
Anti- CBS	Abcam	Cat# ab135626
Anti- SQR	Abcam	Cat# ab118772
Anti- CGL	Proteintech	Cat# 12217–1-AP
Anti- 3MST	Atlas antibodies	Cat# HPA001240
Anti- LC3B	Novus Biologicals	Cat# B100–2220
HRP conjugated goat antibodies against rabbit	Dako	Cat# P044801–2
Bacterial and Virus Strains
N/A
Biological Samples
N/A
Chemicals, Peptides, and Recombinant Proteins
Compound library: Harvard ICCB Known Bioactive collection	ICCB-Longwood	https://iccb.med.harvard.edu/known-bioactives-collection
P3	Calbiochem	Cat# 534329
SF-7 AM	Tocris	Cat# 4943
HSip-1 DA	Dojindo	Cat# SB22–10
SSP4	Dojindo	Cat# SB10–10
Na2S	Sigma-Aldrich	Cat# 407410
AOAA	Cayman	Cat# 28298
Etoposide	Sigma-Aldrich	Cat# E1383
NH4Cl	Sigma-Aldrich	Cat# 254134
Oligomycin	Sigma-Aldrich	Cat# 75351
FCCP	Sigma-Aldrich	Cat# C2920
Tunicamycin	Sigma-Aldrich	Cat# T7765
2-Deoxy-D-glucose (2DG)	Sigma-Aldrich	Cat# D8375
Dexamethasone	Sigma-Aldrich	Cat# D4902
Irinotecan	Selleckchem	Cat# S1198
Trifluridine	Selleckchem	Cat# S1778
Teniposide	Selleckchem	Cat# S1787
Bleomycin	Selleckchem	Cat# S1214
DL-Propargyl Glycine (PAG)	Cayman	Cat# 10010948
AP39	Cayman	Cat# 17100
Sildenafil	Tocris	Cat# 3784
Z-VAD-FMK	Tocris	Cat# 2163/1
brefeldin A	Tocris	Cat# 1231/5
MG132	Tocris	Cat# 1748/5
Chloroquine	Invivogen	Cat# tlrl-chq
Bortezomib	LC laboratories	Cat# B-1408
Rapamycin	EMD Millipore	Cat# 553210
Critical Commercial Assays
CyQUANT Direct Cell Proliferation Assay Kit	Invitrogen	Cat# C7026
Apo-ONE Homogeneous Caspase-3/7 kit	Promega	Cat# G7792
Deposited Data
N/A
Experimental Models: Cell Lines
HeLa cells	ATCC	Cat # ATCC CCL-2
Primary mouse embryonic fibroblast (MEF) cells from C57/BL6 mice	This study	N/A
Primary CGL WT and KO mouse tail dermal fibroblast cells	This study	N/A
Mouse circulating leukocytes	This study	N/A
Mouse bone marrow cells	This study	N/A
WT and Parp-1−/− immortalized MEF cells	From the lab of Dr. Samuel Wilson	(de Murcia et al., 1997)
WT and Atg5−/− immortalized MEF cells	From the lab of Gokhan Hotamisligil	(Yang et al., 2010)
WT and Atg7−/− immortalized MEF cells	From the lab of Gokhan Hotamisligil	(Yang et al., 2010)
WT and Atf4−/− immortalized MEF cells	From the lab of Dr. Craig Thompson	(Han et al., 2013)
Immortalized MEF cells expressing GFP-LC3	From the lab of Dr. Craig Thompson	(Cheong et al., 2011)
WT and AMPKα1−/−/α2−/− immortalized MEF cells	From the lab of Dr. Massimo Loda	(Zadra et al., 2014)
Experimental Models: Organisms/Strains
Cx mouse	Generated in the lab of James Mitchell	(Brace et al., 2013)
CGL WT and KO mouse	International Knockout Mouse Consortium	Cat #MGI:5435787
C57BL6 mice	Jackson Lab	Cat #000664
Oligonucleotides
Mouse CBS FP: GGGACAAGGATCGAGTCTGGA	N/A	N/A
Mouse RP: AGCACTGTGTGATAATGTGGG	N/A	N/A
Mouse CGL FP: TTGGATCGAAACACCCACAAA	N/A	N/A
Mouse CGL RP: AGCCGACTATTGAGGTCATCA	N/A	N/A
Mouse 3MST FP: TCACAGCCGCTGAAGTTACTG	N/A	N/A
Mouse 3MST RP: CAGCATGTGGTCGTAGGGG	N/A	N/A
Mouse SQR FP: CCCGGCTCTTTGCCTGTTT	N/A	N/A
Mouse SQR RP: CCAGCACCTCATAGTGGTTCTT	N/A	N/A
Mouse β-actin FP: GTGACGTTGACATCCGTAAAGA	N/A	N/A
Mouse β-actin RP: GCCGGACTCATCGTACTCC	N/A	N/A
Recombinant DNA
N/A
Software and Algorithms
Image J	National Institute of Health	https://imagej.nih.gov
GraphPad Prism v 8.4.1	GraphPad Software	https://www.graphpad.com
MetaXpress 6	Molecular Devices	https://mdc.custhelp.com
FlowJo	BD Biosciences	https://www.flowjo.com
Other
N/A