Fig.2.
Surface expressions of the T cell determinants and TanCAR molecule. A. Expression of cell surface markers associated with T cells as gated on CD3+CAR+ cells by flow cytometry. B. Detection of the surface expression of the TanCAR using a CD123 Fab-specific antibody and specific FRβ protein on THP1 leukaemia cells (top) and using anti-Fab and FRβ protein on tandem CAR-T cells (bottom). FRβ; Folate receptor beta and CAR; Chimeric antigen receptor