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. Author manuscript; available in PMC: 2022 Jan 11.
Published in final edited form as: Cancer Cell. 2020 Dec 17;39(1):109–121.e5. doi: 10.1016/j.ccell.2020.11.004

Figure 2. Exo1 is essential for innate sensing signaling in Mlh1−/− 4T1 cells.

Figure 2.

(A) Depletion of Exo1 reduces cytosolic DNA accumulation in Mlh1−/− cells regardless of IR treatment. (B) Western blots showing reduced DNA breaks and pSTAT1 when Exo1 was depleted from Mlh1−/− cells. A non-specific band detected by an Exo1 antibody is indicated by an asterisk. (C) Quantification of the relative γH2AX levels in Mlh1 knockout and Mlh1-Exo1 double knockout (Dbl KO) cells. (D) Western blots showing that Exo1 knockout abolishes IR-induced STING activation. (E) Quantification of relative pSTING levels in Mlh1-knockout and Mlh1-Exo1 Dbl KO cells. (F) qRT-PCR analysis showing that Exo1 depletion suppressed expression of Isg15. Data represent the mean ± SEM of 3 independent experiments (A, C and E) or 3 replicates (F). P value was calculated using one-way ANOVA. ****, p<0.0001. See also Figure S2.