|
Antibodies
|
| Purified mouse anti- human MLH1 Antibody |
BD Bioscience |
Cat#550838; RRID:AB_2297859 |
| cGAS (D3O8O) Rabbit mAb (Mouse specific) |
Cell Signaling |
Cat# 31659; RRID:AB_2799008 |
| STING (D2P2F) Rabbit mAb |
Cell Signaling |
Cat# 13647; RRID:AB_2732796 |
| Phospho-STAT1 (Tyr701) (58D6) Rabbit mAb |
Cell Signaling |
Cat# 9167; RRID:AB_561284 |
| Phospho-STING (Ser365) (D8F4W) Rabbit mAb |
Cell Signaling |
Cat# 72971; RRID:AB_2799831 |
| Anti-mouse IgG (H+L), F(ab’)2 Fragment (Alexa Fluor 555 Conjugate) antibody |
Cell Signaling |
Cat# 4409; RRID:AB_1904022 |
| Anti-rabbit IgG (H+L), F(ab’)2 Fragment (Alexa Fluor® 488 Conjugate) antibody |
Cell Signaling |
Cat# 4412; RRID:AB_1904025 |
| Anti-rabbit IgG (H+L), F(ab) 2 Fragment (Alexa Fluor 555 Conjugate) antibody |
Cell Signaling |
Cat# 4413; RRID:AB_10694110 |
| α Tubulin antibody (B-5-1-2) |
Santa Cruz |
Cat# sc-23948; RRID:AB_628410 |
| Beta Actin mAb (8H10D10) |
Novus Biologicals |
Cat# NBP1-47423; RRID:AB_10010376 |
| Phospho-RPA32 (S4/8) antibody (A300-245A) |
Bethyl laboratories |
Cat# A300-245A; RRID:AB_210547 |
| BrdU (5-bromo-2-deoxyuridine) antibody |
Millipore Sigma |
Cat# MAB3510; RRID:AB_2314031 |
| Anti-Replication Protein A, clone RPA34-20 antibody) |
Millipore Sigma |
Cat# MABE285; RRID:AB_11205561 |
| Phospho-Histone H2AX (Ser139) mouse mAb |
Millipore Sigma |
Cat# 05-636-I; RRID:AB_2755003 |
| Goat Anti-Rabbit IgG Antibody, (H+L) HRP conjugate |
Millipore Sigma |
Cat# AP307P; RRID:AB_92641 |
| Goat Anti-Mouse IgG & IgM Antibody, HRP conjugate |
Millipore Sigma |
Cat# AP130P; RRID:AB_91266 |
| Anti-human Exo1 Antibody |
This paper |
Custom-made |
| Phospho-Exo1 (S746) Antibody |
(Li et al., 2019) |
N/A |
|
Chemicals, Peptides, and Recombinant Proteins
|
| ProLong™ Diamond Antifade Mountant with DAPI |
Fisher Scientific |
Cat# P36962
|
| Pico488 dsDNA quantification reagent |
Lumiprobe |
Cat# 12010 |
|
Critical Commercial Assays
|
| Mouse IFN Beta ELISA Kit |
PBL Assay Science |
Cat# 42410 |
| M-MuLV Reverse Transcriptase |
New England Biolabs |
Cat# M0253S |
| SsoAdvanced Uni SYBR Grn Supmix |
Bio-Rad |
Cat# 1725272 |
| Taqman™ Fast Advanced master mix |
Thermo Fisher |
Cat# 4444557 |
| Bio-Rad protein assay dye reagent concentrate |
Bio-Rad |
Cat#5000006 |
| jetPRIME™ Transfection reagent |
PolyPlus |
#114-07 |
|
Deposited Data
|
| Raw and analyzed data |
This paper |
Mendeley data: Reserved DOI: 10.17632/mrg4443z7m.1
|
| Experimental Models: Cell Lines |
| 4T1 |
ATCC |
Cat# CRL-2539; RRID:CVCL_0125 |
| HeLa |
ATCC |
Cat# 60005; RRID:CVCL_0030 |
| HCT116 |
ATCC |
Cat# KCB 200706YJ; RRID:CVCL_0291 |
| ER-AsiSI-U2OS |
(Zhou et al., 2014) |
N/A |
|
Oligonucleotides
|
| Mouse Isg15 forward: 5’-GAGCTAGAGCCTGCAGCAAT-3’ |
This paper |
N/A |
| Mouse Isg15 reverse: 5’-TCACGGACACCAGGAAATCG-3’ |
This paper |
N/A |
| Mouse Irf7 forward: 5’-TTGGGCAAGACTTGTCAGCA-3’ |
This paper |
N/A |
| Mouse Irf7 reverse: 5’-ATACCCATGGCTCCAGCTTC-3’ |
This paper |
N/A |
| Mouse Gapdh forward: 5’-CAACTGCTTAGCCCCCCTGG-3’ |
This paper |
N/A |
| Mouse Gapdh reverse: 5’-GCAGGGTAAGATAAGAAATG-3’ |
This paper |
N/A |
| DSB1-335 forward: 5’-GAATCGGATGTATGCGACTGATC-3’ |
This paper |
N/A |
| DSB1-335 reverse: 5’-TTCCAAAGTTATTCCAACCCGAT-3’ |
This paper |
N/A |
| DSB1-335 probe: 6FAM-CACAGCTTGCCCATCCTTGCAAACC-TAMRA |
This paper |
N/A |
| DSB1-1618 forward: 5’-TGAGGAGGTGACATTAGAACTCAGA-3’ |
This paper |
N/A |
| DSB1-1618 reverse: 5’-AGGACTCACTTACACGGCCTTT-3’ |
This paper |
N/A |
| DSB1-1618 probe: 6FAM-TTGCAAGGCTGCTTCCTTACCATTCAA-TAMRA |
This paper |
N/A |
| DSB1-3500 forward: 5’-TCCTAGCCAGATAATAATAGCTATACAAACA-3’ |
This paper |
N/A |
| DSB1-3500 reverse: 5’-TGAATAGACAGACAACAG-3’ |
This paper |
N/A |
| DSB1-3500 probe: 6FAM-ACCCTGATCAGCCTTTCCATGGGTTAAG-TAMRA |
This paper |
N/A |
|
Recombinant DNA
|
| pLentiCRISPR v2 |
(Sanjana et al., 2014) |
Addgene Plasmid Cat#52961 |
| pSpCas9(BB)-2A-GFP (PX458) |
(Hmelo et al., 2015) |
Addgene Plasmid Cat #48138 |
| pCMV6-Entry-mouse Mlh1
|
Origene |
Cat#: MR210511 |
| pEGFP-N1-Exo1
|
This paper |
N/A |
| pLVX-CMV-human MLH1
|
This paper |
N/A |
|
Software and Algorithms
|
| GraphPad Prism software 8.0 |
GraphPad Software |
N/A |
| Carl Zeiss Axiovision software v4.91 |
Carl Zeiss |
N/A |
| Carl Zeiss ZEN lite software |
Carl Zeiss |
N/A |
| ImageJ software |
NIH |
N/A |
| the LAS X software |
Leica |
N/A |
