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Calculation of validation parameters for the screening protocol
| Step | Average | SD | RSD (%) | LOD | LOQ |
|---|---|---|---|---|---|
| Fluorescence signal measurement (RFU) | 34,791.44 | ± 753.31 | 2.16 | 3155.52 | 3497.40 |
| SAK activity assay (IU/mg) | 184.87 | ± 3.18 | 1.72 | 0.68 | 27.53 |
| Number of double transformed clones | 107.33 | ± 2.5 | 2.32 | – | – |
| Obtaining a clone in each well of 96-microplate | 1 | ± 0.00 | 0 | – | – |
SD: standard deviation; RSD: relative standard deviation; LOD: limit of detection; LOQ: limit of quantification
