FIGURE 4.

Effects of ZFP36L2 or RAB13 overexpression or knockdown on the expression of autophagic proteins in SW1088 cells; (A) SW1088 cells transfected with vector, si‐ZFP36L2, si‐RAB13, c‐ZFP36L2, c‐RAB13, autophagic proteins were quantified by Western blot. All data were representative of at least three independent experiments. ns, no significance; p* < 0.05, **p < 0.01, ***p < 0.001. (B) The expression and location of LC3 were detected by immunofluorescence. Scale bar = 20 µm. (C) After SW1088, cells were transfected with si‐RAB13 and c‐RAB13 for 24 h in the presence or absence of rapamycin (10 nM). Then, SW1088 cells was transfected with GFP/mRFP‐LC3 plasmid, representative images and quantitative analysis of LC3 puncta were shown. Scale bar = 20 µm. Merge compared with the NC group, # p < 0.05, ## p < 0.01, ### p < 0.001, ns, no significance; RFP compared with the NC group, *p < 0.05, ** p < 0.01, *** p < 0.001, ns, no significance