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. 2021 Nov 1;54(12):e13146. doi: 10.1111/cpr.13146

FIGURE 2.

FIGURE 2

Overexpression of LRRC8A induced a voltage‐dependent Cl− current under isotonic conditions. (A, B) Representative traces of current (A) and I–V curves (B) in LRRC8A‐expressing adenovirus‐transfected BASMCs before and after DIDS treatment in isotonic solution (n = 6). (C, D) Representative traces of current (C) and I–V curves (D) when extracellular Cl− concentration was changed from 115 to 45 mM in LRRC8A‐expressing adenovirus‐transfected BASMCs (n = 6). (E–G) Representative traces of current (E), average current densities measured at ±80 mV (F), and I–V curves (G) in LRRC8A overexpression adenovirus‐transfected BASMCs showing the evoked voltage‐dependent Cl− current at isotonic condition were inhibited after exposure to hypertonic solution (n = 5, *p < 0.05 VS ISO). (H) Cl− concentration was measured in the indicated solutions in Control or LRRC8A‐expressing adenovirus (Ad‐LRRC8A)‐transfected BASMCs. (I) Quantification of Cl− concentration from (H) in the indicated solutions (n = 6, *p < 0.05)