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. 2021 Nov 1;54(12):e13146. doi: 10.1111/cpr.13146

FIGURE 4.

FIGURE 4

LRRC8A increases proliferation in BASMCs. BASMCs were transfected with LRRC8A siRNA, LRRC8A‐expressing adenovirus (Ad‐LRRC8A), or the respective negative control (Negative and Ad‐Lacz) for 48 h, the cell viability was measured by CCK‐8 assay (A); cell cycle transition was detected by flow cytometric analysis (B) (n = 6, *p < 0.05). (C, D) Representative western blot images (C) and the quantification graphs (D) of Cyclin D1, Cyclin E, CDK2 expression in LRRC8A siRNA‐treated BASMCs. Ctrl, control; Neg, negative control; si‐RNA, LRRC8A siRNA (n = 6, *p < 0.05 VS Ctrl or Neg). (E, F) Representative western blot images (E) and the quantification graphs (F) of Cyclin D1, Cyclin E, and CDK2 expressions in LRRC8A‐expressing adenovirus‐transfected BASMCs. Ctrl, control; Ad‐LacZ, LacZ control adenovirus; Ad‐LRRC8A, LRRC8A overexpression adenovirus (n = 5, *p < 0.05 VS Ctrl or Ad‐LacZ)