FIGURE 3.

Preoperative examination of x‐rays from patients with aseptic loosening and the analysis of related mRNA expression in peri‐implant bone tissue of patients. (A) Preoperative radiographs of patients with aseptic loosening. The red arrow represents the site from where we collected the specimens. Control: developmental dysplasia of the hip required THA. Unloose: unloose specimens; Case1‐4: loose specimens. (B) The mRNA expression of osteogenic marker genes Runx2, Ocn, β‐Catenin, Osterix, Col1a1, and Opg in bone tissue of these patients. (C) The mRNA expression of ferroptosis‐related genes, Gpx4, Slc7a11, and Ptgs2, in bone tissue from these patients. Data represent mean ± SD of triplicate independent experiments. ^{*, #} indicates p < 0.05, ^{**, ##} indicates p < 0.01, ^ns indicates not significant