Table 3.
Recommended input and plating conditions for mixed enteroid monolayers
| Well format |
Number of cells /well |
|||||||
|---|---|---|---|---|---|---|---|---|
| Ratio 1:1 |
Ratio 1:2 |
Ratio 1:4 |
||||||
| Type | Size | Volume | A | B | A | B | A | B |
| 384 (Greiner) | 0.10 cm2 | 50 μL | 3000 | 3000 | 3000 | 1500 | 3000 | 750 |
| 96 (Greiner) | 0.34 cm2 | 100 μL | 10000 | 10000 | 10000 | 5000 | 10000 | 2500 |
| 96 (IBIDI) | 0.56 cm2 | 150 μL | 15000 | 15000 | 15000 | 7500 | 15000 | 3750 |