Enteroid plating medium

Reagent	Stock concentration	Final concentration	Amount
Basic culture medium	n/a	n/a	38.77 mL
B-27	50×	2%	1 mL
N-Acetyl-L-Cysteine	500 mM	1.25 mM	125 μL
R-spondin1 CM	n/a	10%	5 mL
Noggin CM	n/a	10%	5 mL
hEGF	0.5 mg/mL	50 ng/mL	5 μL
CHIR-99021	3 mM	3 μM	50 μL
Y-27632	10 mM	10 μM	50 μL
Total			50 mL

Prepare in a sterile environment, store at 4°C for up to 2 weeks.