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. 2021 Oct 18;38(12):3175–3193. doi: 10.1007/s10815-021-02314-x

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — The S-sulfhydrated proteome of human sperm. (A) Detection of S-sulfhydrated proteins in human and mouse sperm. The lysates were prepared from pooled samples of human sperm (from ten fertile men) and mouse sperm (from three male mice), next subjected to the modified biotin-switch assay. The numerous sulfhydrated proteins were detected with anti-biotin antibody (left) or with silver staining (right). (B) LC-MS/MS of a subset of the S-sulfhydrated proteins in (A) identifies top notable S-sulfhydrated proteins, including GAPDH, GSTM3, and H3. (C) Biological processes enriched in the S-sulfhydrated proteins of human sperm. (D) Vann diagram depicting the relationship of sulfhydrated proteins and ROS-associated proteins of human sperm.