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. 2021 Oct 18;38(12):3175–3193. doi: 10.1007/s10815-021-02314-x

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Analysis of expression of sH3 and sH3.3 in spermatogenesis and effect of RA and NaHS on level of sH3 in germ cells. (A) Spermatognia (SG), pachytene spermatocytes (pacSC), round spermatids (rST), and elongating/condensed spermatids (eST) were isolated and characterized as described in “Experimental procedures.” These germ cells, together with sperm from caput epididymis (SPM (cau)) and from cauda epididymis (SPM (cau)), were applied for preparation for protein lysates, subjected for measurement of expression of sH3 and sH3.3. (B) The incubated rST were treated with two indicated doses of RA for 24 h, then subjected for analysis of sH3.3 expression. (C) The incubated pieces of testis were treated with the indicated concentrations of NaHS for 24 h, and next subjected for measurement of expression of sH3.3. The experiments were repeated independently for three (for B and C) to four (for A) times. Error bar denotes mean ± SEM. *P < 0.05 and **P < 0.01.