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. 2021 Nov 29;12:739219. doi: 10.3389/fimmu.2021.739219

Figure 4.

Figure 4

MIR337-3p depressed antimicrobial response of VDR-related CYP27B1, DEFB4A, and CAMP pathways. (A) Transfection of MIR337-3p mimics downregulated gene expression of DEFB4A, CAMP, and CYP27B1 in THP-1 cells at 24 h after transfection, respectively (left panel). And transfection of MIR337-3p mimics depressed CYP27B1 protein expression in THP-1 cells (right panel). (B) The gene expressions of DEFB4A, CAMP, and CYP27B1 were higher in MIR337-3p inhibitor-transfected THP-1 cells than those in inhibitor-control-transfected cells, respectively. (C) shows relative gene expression levels of CAMP and CYP27B1 in MIR337-3p mimic- and control-transfected cells in stimulation of vitamin 1,25-(OH)2D3, respectively. (D) shows representative results in Western blot (WB) indicating that MIR337-3p inhibitor enhances CYP27B1 protein expression in THP-1 cells in stimulation of vitamin 1,25-(OH)2D3. (E) shows BCG CFU counts in vitamin 1,25-(OH)2D3-treated THP-1 cells, which were transfected with MIR337-3p mimics or inhibitors and their controls, respectively. Data are represented as mean ± standard errors. *P<0.05, **P < 0.01 and ***P < 0.001.