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Heterologous expression of GmMTP8.1 in different yeast mutants. The yeast mutants Δpmr1, Δzrc1, Δcup2, Δycf1, and Δcot1 harboring the pYES2 empty vector or the pYES2-GmMTP8.1 construct were used. Yeast cells (10 μl) with OD600 of 0.2 and four serial dilutions (10-fold) were spotted in SC-U/Gal medium added with or without 5 mM MnSO4, 15 mM ZnSO4, 100 μM CuSO4, 50 μM CdCl2, and 2 mM CoCl2 for 2 day at 30°C.
