FIGURE 8.

Growth and Mn concentration of Arabidopsis overexpressing GmMTP8.1. (A) Phenotype of Arabidopsis grown with different Mn treatments. (B) Shoot fresh weight. (C) Root fresh weight. (D) Plant fresh weight. (E) Mn concentration in shoot. (F) Mn concentration in root. (G) Mn concentration in plant. Seven-day-old Arabidopsis seedlings were transferred to a solid Murashige and Skoog (MS) medium containing 0.1, 2, or 4 mM MnSO₄. Arabidopsis plants treated with 0.1 mM MnSO₄ were set as the control. After 7 days of Mn treatment, fresh weight and Mn concentration were determined. WT represents the wild type Arabidopsis. OE1 and OE2 are two transgenic lines overexpressing GmMTP8.1. Each bar represents the mean value of three independent replicates with standard error. White, gray, and black bars represent WT, OE1, and OE2, respectively. Asterisks indicate significant differences between the wild type and the overexpression lines with the same Mn treatment. *, 0.01 < P < 0.05. **, 0.001 < P < 0.01. DW, dry weight. Scale bar is 2 cm.