Table 3.
Genotype frequency with percentages shown in parenthesis of the nine possible F167Y/F200Y genotype combinations observed for the parental isolates and genetic cross generations using pyrosequencing on individual larvae; where F3 generations have results for untreated and post fenbendazole (BZ) drug treatment.
| Genotype | MHco3 (ISE) | MHco18 (UGA2004) | MHco3/18.F1 | MHco3/18.F2 | MHco3/18.F3 | MHco3/18.F3.BZ | MHco18/3.F1 | MHco18/3.F2 | MHco18/3.F3 | MHco18/3.F3.BZ | |
|---|---|---|---|---|---|---|---|---|---|---|---|
| SS-SS | TT-TT | 84 (100.0) | 0 (0.0) | 0 (0.0) | 23 (33.3) | 25 (32.5) | 2 (2.6) | 0 (0.0) | 17 (21.3) | 17 (30.9) | 0 (0.0) |
| SS-SR | TT-AT | 0 (0.0) | 0 (0.0) | 66 (81.5) | 25 (36.2) | 26 (33.8) | 22 (29.0) | 56 (63.6) | 33 (41.3) | 12 (21.8) | 15 (18.5) |
| SS-RR | TT-AA | 0 (0.0) | 36 (42.4) | 0 (0.0) | 8 (11.6) | 9 (11.7) | 35 (46.1) | 0 (0.0) | 5 (6.3) | 7 (12.7) | 29 (35.8) |
| RS-SS | AT-TT | 0 (0.0) | 0 (0.0) | 3 (3.7) | 1 (1.5) | 4 (5.2) | 2 (2.7) | 10 (11.4) | 3 (3.8) | 6 (10.9) | 0 (0.0) |
| RS-RS | AT-AT | 0 (0.0) | 15 (17.7) | 12 (14.8) | 7 (10.1) | 11 (14.3) | 8 (10.5) | 22 (25.0) | 8 (10.0) | 10 (18.2) | 14 (17.3) |
| RS-RR | AT-AA | 0 (0.0) | 28 (33.0) | 0 (0.0) | 5 (7.3) | 1 (1.3) | 6 (7.9) | 0 (0.0) | 13 (16.3) | 2 (3.6) | 14 (17.3) |
| RR-SS | AA-TT | 0 (0.0) | 2 (2.4) | 0 (0.0) | 0 (0.0) | 0 (0.0) | 0 (0.0) | 0 (0.0) | 0 (0.0) | 1 (1.8) | 2 (2.5) |
| RR-RS | AA-AT | 0 (0.0) | 1 (1.2) | 0 (0.0) | 0 (0.0) | 1 (1.3) | 1 (1.3) | 0 (0.0) | 1 (1.3) | 0 (0.0) | 4 (4.9) |
| RR-RR | AA-AA | 0 (0.0) | 3 (3.5) | 0 (0.0) | 0 (0.0) | 0 (0.0) | 0 (0.0) | 0 (0.0) | 0 (0.0) | 0 (0.0) | 3 (3.7) |