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. 2021 Dec 13;7(4):00247-2021. doi: 10.1183/23120541.00247-2021

FIGURE 1.

FIGURE 1

Effect of inflammatory mediators on the expression of airway chloride channels and transporters in differentiated BCi-NS1.1 cells. Western blot of endogenous a) cystic fibrosis transmembrane conductance regulator (CFTR) (∼180 kDa), b) transmembrane protein 16A (TMEM16A) (∼120 kDa) and c) solute carrier family 26 member SLC26A4 (∼85 kDa) expression following exposure to cytokines. GAPDH (∼36 kDa) was used as a loading control. For each cytokine, representative blots are shown. Dashed lines indicate lanes run on the same gel but noncontiguous. IL: interleukin; TNF: tumour necrosis factor; Con: control. d) CFTR, TMEM16A and SLC26A4 protein expression levels were detected by Western blotting, quantified by densitometry and normalised to the loading control and control untreated cells. Data are presented as mean±sem (n=3–4). *: p<0.05 versus control, unpaired t-test. e) SLC26A9 mRNA expression levels following exposure to cytokines, quantitatively measured using quantitative reverse transcriptase PCR and normalised to the housekeeping gene GAPDH. Fold change values are mean±sem, relative to control untreated cells (n=3–4). *: p<0.05 versus control, unpaired t-test. f) Contribution of CFTR, TMEM16A, SLC26A4 and SLC26A9 for total chloride channels/transporters expression following exposure to each cytokine. Values were calculated by dividing the fold change of each channel's protein/mRNA expression levels shown in d) and e) by the sum of all.