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. 2021 Nov 29;8:766073. doi: 10.3389/fmed.2021.766073

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Copyright © 2021 Zhang, Qi, Li, Zhang, Shi, Xu and Zhu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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LPS induces apoptosis mainly in podocytes and proximal tubular cells, but not in distal tubular cells. (A) Quantitative PCR analysis of the podocytes markers (NPHS1, NPHS2, PODXL), proximal tubular marker (HNF4A), and distal tubular markers (GATA3, CDH1). ns, non-significant, ^*P < 0.05, ^**P < 0.01, ^***P < 0.001. (B) Expression pattern of the podocyte marker SYNPO (Green) as well as cleaved Caspase-3 (Red) in the control, LPS, and LPS + MP treatment group. (C) Expression pattern of the proximal tubular marker LTL (Green) as well as cleaved Caspase-3 (Red). (D) Expression pattern of the distal tubular marker ECAD (Green) as well as cleaved Caspase-3 (Red). DAPI is stained with blue fluorescence. Scar bar = 100 μm.