Figure 8.

Reactive oxygen accumulation and related gene expression and hypersensitive response in the TaMYB29 knockdown AvS+Yr10 wheat leaves. (A) The reactive oxygen species and cell necrosis in wheat leaves in response to Pst observed under a fluorescence microscope. (B) The reactive oxygen species areas were stained with DAB and measured microscopically at 24, 48, and 120hpi. H₂O₂ accumulation was significantly reduced in TaMYB29 silenced leaves compared to the control. (C) Transcriptional level of ROS-related gene TaCAT in AvS+Yr10 after TaMYB29-scilencing at four time points relative to the expression in BSMV:00. (D) Cell death was measured microscopically because of the auto-fluorescence of necrotic cells at 48 and 120hpi. The area of green auto-fluorescence reduced in TaMYB29-silenced wheat plant. Pictures were taken under an epifluorescence or light microscopy at 24, 48, and 120hpi, respectively. BSMV:00 is the control group and BSMV:TaMYB29 is the experimental group in which TaMYB29 gene was silenced. U, urediniospore; GT, germ tube; NC, necrotic cell; SV, substomatal vesicle; IH, initial hyphae; HMC, haustorial mother cell; and SH, secondary hyphae. Bars=20μm. Error bars represent standard deviation among three biological replications. Student’s t-tests were used to for the statistical analyses and the asterisks (∗) and (∗∗) indicate a significant difference between particular hpi and controls with a value of p<0.05 and 0.01, respectively.