Table 2.

Bioreactor settings (exemplarily for a 150 mL DASbox bioreactor)

Stirring	Temperature	Gassing	pH	DO	Perfusion
80 rpm (exact calculation based on Equations 13 + 14) ccw	37°C	0.9 sL/h (reactor volume × 6), 21% O2, 5% CO2	7.1	40%	see Table 3