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. 2021 Jan 27;71(1):78–87. doi: 10.1136/gutjnl-2020-323347

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© Author(s) (or their employer(s)) 2022. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/.

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The effect of abnormal vitamin A metabolism was transferable to testis. (A) The testicular vitamin A levels of the ND and HED fed groups were measured (n=6). (B) Serum vitamin A levels of the ND, MED and HED-fed groups were examined (n=6). (C) The representative images of RBP4 and RDH10 immunofluorescent (IF) staining in sections of testicular tissue. Scale bar=50 μm. (D) The IF staining for STRA8 and SYCP3 is shown together with MVH in representative sections of testicular tissue. Scale bar=25 μm. (E) Western blot of STRA8, RDH10, MVH, GAPDH and β-ACTIN in each indicated group. (F) Quantification protein levels of (a) STRA8, (b) RDH10 and (c) MVH. HED, high-energy diet; MED, medium-energy diet.