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. 2021 Jan 27;71(1):78–87. doi: 10.1136/gutjnl-2020-323347

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© Author(s) (or their employer(s)) 2022. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

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Faecal transplants of HED microbiota exhibited disrupted spermatogenesis induced by a notable reduction in levels of vitamin A and RDH10. (A) Study design diagram of the faecal transplant experiment. (B) Vitamin A level measured using an ELISA kit (n=6 for ND-FMT; n=10 HED-FMT). (C) The qPCR analysis of Ruminococcaceae_NK4A214_group abundance (n=9). (D) H&E staining of ND-FMT and HED-FMT testicular tissue. (E) Percentage of abnormal tubules in testicular tissue from each group (n=7). (F) Representative IF staining images of STRA8 and RDH10 in sections of testicular tissue. Scale bar=50 μm. (G) Western blotting of STRA8, RDH10, MVH and β-ACTIN in each indicated group. (H) Quantification protein levels of (a) STRA8, (b) RDH10 and (c) MVH. FMT, faecal microbiome transplantation; HED, high-energy diet; IF, immunofluorescent.