FIG. 6.
Binding of in vitro-translated 35S-labeled GR to myGR immunoprecipitated from whole-cell extracts requires amino acids 511 to 539. Immunoprecipitates from whole-cell extracts prepared from Sf7 cells expressing myGR [Sf7 (GR+); lanes 4 to 6] or control cells (Sf7, lanes 7 to 9) as indicated were tested for binding to the in vitro-translated GRs, whose composition is illustrated schematically at the top. All samples were treated with 10−6 M Dex. GR binding is compared to 10% of the input from the in vitro translations shown in lanes 1 to 3.