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Free energy profiles for close (solid circles) to open (open circles) transitions in SUR1. R is the distance between centers of mass (COM) of NBD1 and NBD2 domains (a). Distributions of probabilities for finding SUR structures of the given NBD1-NBD2 distance (b). The probability density function P(R) in each case is normalized. Errors of free energy profiles (convergence) are shown as the largest deviation of 130 ns and consecutive profiles from the presented 170 ns plots.
